Genome Biology - Most viewed articlesMost viewed articles in last 30 days from Genome Biology (ISSN 1465-6906) published by BioMed CentralAccurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes- June 18, 2002 Background: Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large dynamic range of this methodology, the requirements for a proper internal control gene for normalization have become increasingly stringent. Although housekeeping gene expression has been reported to..http://genomebiology.com/2002/3/7/research/0034 |