BMC Molecular Biology - Latest articlesThe latest articles from BMC Molecular Biology (ISSN 1471-2199) published by BioMed CentralSystemic RNAi mediated gene silencing in the anhydrobiotic nematode Pangrolaimus superbus- June 19, 2008 Background: Gene silencing by RNA interference (RNAi) is a powerful tool for functional genomics. A large number of high-throughput RNAi screens have been carried out in RNAi-amenable organisms, most notably in the nematode Caenorhabditis elegans. Although RNAi was first described in C. elegans, several nematode species are unable to mount an RNAi response when exposed to exogenous double stranded RNA (dsRNA). These include the satellite model organisms Pristionchus pacificus and Oscheius..http://www.biomedcentral.com/1471-2199/9/58 TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function- June 12, 2008 Background: The tumour suppressor protein p53 is a sequence specific DNA-binding transcription regulator, which exerts its versatile roles in genome protection and apoptosis by affecting the expression of a large number of genes. In an attempt to obtain a better understanding of the mechanisms by which p53 transcription function is regulated, we studied p53 interactions. Results: We identified BIP2 (Bric-a-brac interacting protein 2), the fly homolog of TAF3, a histone fold and a plant...http://www.biomedcentral.com/1471-2199/9/57 Identification of a candidate alternative promoter region of the human Bcl2L11 (Bim)gene- June 12, 2008 Background: Despite the importance of the BCL2L11 (BIM) protein in various apoptotic processes in development and disease, little is known of the promoter structure of the human BCL2L11 locus and of the cis-acting elements regulating expression of the human gene. Results: In the search for novel promoter sequences in the human BCL2L11 locus, we have identified previously unrecognized genomic sequences displaying promoter activity and E2F responsiveness, and driving the expression of BCL2L11...http://www.biomedcentral.com/1471-2199/9/56 Ltr retrotransposons and the evolution of dosage compensation in Drosophila- June 4, 2008 Background: Dosage compensation in Drosophila is the epigenetic process by which the expression of genes located on the single X-chromosome of males is elevated to equal the expression of X-linked genes in females where there are two copies of the X-chromosome. While epigenetic mechanisms are hypothesized to have evolved originally to silence transposable elements, a connection between transposable elements and the evolution of dosage compensation has yet to be demonstrated. Results: We show...http://www.biomedcentral.com/1471-2199/9/55 A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids- June 4, 2008 Background: Real-time PCR techniques are being widely used for nucleic acids analysis, and one limitation of current mainly employed real-time PCR is the high cost of the labeled probe for each target molecule. Results: One real-time PCR technique employing attached universal duplex probes (AUDP) is developed, which is supposed to have the advantage of generating fluorescence by probe hydrolysis and strand displacement over current real-time PCR methods. AUDP is one set of universal duplex...http://www.biomedcentral.com/1471-2199/9/54 |