BMC Chemical Biology - Latest articlesThe latest articles from BMC Chemical Biology (ISSN 1472-6769) published by BioMed CentralDevelopment of ERK Activity Sensor, an in vitro, FRET-based sensor of Extracellular Regulated Kinase activity- July 5, 2005 Background: Study of ERK activation has thus far relied on biochemical assays that are limited to the use of phospho-specific antibodies and radioactivity in vitro, and analysis of whole cell populations in vivo. As with many systems, fluorescence resonance energy transfer (FRET) can be utilized to make highly sensitive detectors of molecular activity. Here we introduce FRET-based ERK Activity Sensors, which utilize variants of Enhanced Green Fluorescent Protein fused by an ERK-specific peptide.http://www.biomedcentral.com/1472-6769/5/1 |