BMC Chemical Biology - Latest articlesThe latest articles from BMC Chemical Biology (ISSN 1472-6769) published by BioMed CentralThe effect of amino acid deletions and substitutions in the longest loop of GFP- June 26, 2007 Background: The effect of single and multiple amino acid substitutions in the green fluorescent protein (GFP) from Aequorea victoria has been extensively explored, yielding several proteins of diverse spectral properties. However, the role of amino acid deletions in this protein -as with most proteins- is still unknown, due to the technical difficulties involved in generating combinatorial in-phase amino acid deletions on a target region. Results: In this study, the region I129-L142 of superglo.http://www.biomedcentral.com/1472-6769/7/1 |